This title slide introduces "High-Throughput In Vitro Genotoxicity Testing," focusing on modern methods for efficient safety assessment. It is presented by Noah Banholzer.

High-Throughput In Vitro

Genotoxicity Testing

Modern Methods for Efficient Safety Assessment Noah Banholzer

Source: Noah Banholzer

This agenda slide outlines a presentation on genotoxicity testing, starting with an introduction to its overview and objectives. It then covers current challenges with traditional in vitro methods, high-throughput innovative screening approaches, validation with advantages and case studies, and concludes with key takeaways and future directions.

Presentation Overview

1. Introduction

Overview of genotoxicity testing and objectives

2. Current Challenges

Limitations of traditional in vitro methods

3. High-Throughput Methods

Innovative approaches for efficient screening

4. Validation & Advantages

Verification, benefits, and case studies

5. Conclusion

Key takeaways and future directions Source: High-throughput In Vitro Methods for Genotoxicity Testing

This section header slide introduces "Genotoxicity Testing" as section 01. It highlights foundational concepts and the critical importance of detecting DNA damage in safety assessments.

Introduction to Genotoxicity Testing

01

Introduction to Genotoxicity Testing

Foundational concepts and importance of detecting DNA damage in safety assessments

Source: High-throughput In Vitro Methods for Genotoxicity Testing by Noah Banholzer

The slide "Current Challenges & Needs" outlines original issues in compound testing, including low throughput, high costs, long turnaround times, limited in vivo predictivity, high animal use, and resource intensity. It contrasts these with solutions like scalable in vitro assays, reduced costs and time, improved predictivity, and animal-free testing, illustrated by a high-throughput screening workflow figure.

Current Challenges & Needs

| • Low throughput (few compounds/week)

• High cost per compound
• Long turnaround times
• Limited predictivity for in vivo outcomes
• High animal use
• Resource intensive | [Figure: High-throughput screening workflow]

🔬 Lab pipette 🧫 Cell cultures

• Scalable in vitro assays
• Reduced costs & time
• Improved predictivity
• Animal-free testing |

Source: High-throughput In Vitro Methods for Genotoxicity Testing - Noah Banholzer

High-Throughput In Vitro Methods highlight efficient lab techniques using multi-well plates for parallel testing, automated liquid handling systems, and robotic workflow integration. These enable scalable genotoxicity assays to accelerate research processes.

High-Throughput In Vitro Methods

!Image

• Multi-well plates for parallel testing
• Automated liquid handling systems
• Robotic workflow integration
• Scalable genotoxicity assays

Source: Image from Wikipedia article "High-throughput screening"

The slide "Method Features & Validation" showcases key features of a genotoxicity detection method, including original molecular features from high-throughput assays, statistical metrics like sensitivity and specificity, comprehensive in vitro validation, enzyme assays, molecular endpoints, and accuracy highlights. It uses icons and concise descriptions to highlight robust performance data and validated biomarkers for DNA damage and repair.

Method Features & Validation

Source: High-throughput In Vitro Methods for Genotoxicity Testing

The slide highlights key advantages of the technology, including 95% prediction accuracy matching in vivo results, 10x faster throughput versus traditional methods, 90% cost reduction for per-compound testing, and over 50 validated real-world genotoxicity case studies. These stats demonstrate superior performance and practical validation in a concise format.

Advantages & Case Studies

• 95%: Prediction Accuracy

Matches in vivo results

• 10x: Faster Throughput

Versus traditional methods

• 90%: Cost Reduction

Lower per compound testing

• 50+: Validated Case Studies

Real-world genotoxicity examples

High-throughput in vitro methods accelerate genotoxicity testing with enhanced predictivity, reduced animal use, cost efficiency, and scalability for regulatory and discovery applications. The future involves AI integration and multi-omics expansion, advancing safer chemistry—contact us to integrate these methods today.

Conclusion & Outlook

<ul style='font-size: 24px; line-height: 1.4; color: #333; max-width: 800px; margin: 0 auto;'> <li style='margin-bottom: 12px;'>High-throughput in vitro methods accelerate genotoxicity testing</li> <li style='margin-bottom: 12px;'>Enhanced predictivity, reduced animal use, cost efficiency</li> <li style='margin-bottom: 12px;'>Scalable for regulatory and discovery applications</li> <li style='margin-bottom: 20px;'>Future: AI integration, multi-omics expansion</li> </ul> <div style='text-align: center; margin-top: 40px;'> <svg width='120' height='120' viewBox='0 0 120 120' fill='none' xmlns='http://www.w3.org/2000/svg'> <circle cx='60' cy='60' r='55' fill='#E3F2FD' stroke='#2196F3' stroke-width='3'/> <path d='M60 25 L70 45 H85 L65 55 L75 75 L60 65 L45 75 L55 55 L35 45 H50 Z' fill='#2196F3'/> </svg> <p style='font-size: 28px; font-weight: bold; color: #2196F3; margin-top: 20px;'>Advancing Safer Chemistry</p> <p style='font-size: 20px; color: #666; margin-top: 10px;'>Contact us to integrate these methods today.</p> </div>

Source: High-throughput In Vitro Methods for Genotoxicity Testing