Slide 1 of 21
Slide 1 - The ADAMTS Gene Family of Nematostella vectensis
The ADAMTS Gene Family of Nematostella vectensis
Benjamin Kimelfeld Bollyky Lab Meeting – Feb 19, 2026
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Nv-ADAMTS Family: Evolution and Regeneration in Nematostella
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Redesign and enhance the uploaded presentation titled "Bollyky Lab Meeting Feb-19-2026 – Benjamin Kimelfeld" using a Dark Research Conference Theme. Design Requirements: - Background: deep charcoal / navy gradient - Accent color: neon cyan / teal - Clean, modern, high-contrast scientific aesthetic - Subtle molecular / network texture in corners (low opacity) - Section divider slides with bold glowing titles - Consistent typography (modern sans-serif) - Rounded white containers with soft shadows for figures - Footer: Lab name | Date | Slide number Content Structure (preserve and improve clarity, formatting, and visual hierarchy): 1. Title Slide - The ADAMTS Gene Family of Nematostella vectensis - Benjamin Kimelfeld - Bollyky Lab Meeting – Feb 19, 2026 2. Themes of Presentation - Nematostella as a model organism - Characterization of Nv-ADAMTS genes - Phylogenetics + domain architecture - Development & regeneration expression - Focus on Nv-ADAMTS1, 6, 14, 19 --- Section Divider --- Nematostella vectensis: A Basal Metazoan Model 3. Life Cycle & Morphology 4. Whole-Body Regeneration & Experimental Tools --- Section Divider --- ADAMTS Proteases Background 5. ADAMTS Modular Structure 6. ADAMTS Clades & Vertebrate Functions 7. ADAMTS in Cnidarians – Open Questions --- Section Divider --- Nv-ADAMTS Family Analysis 8. Identifying Nv-ADAMTS Homologues 9. Motif Conservation 10. Phylogenetic Pipeline 11. Shifting ADAMTS Clade Origins --- Section Divider --- Expression Analysis 12. Spatiotemporal Expression Experiments 13. Spatial Expression – 4 Body Parts 14. Spatial Expression – 3 Body Parts 15. Developmental Expression 16. Regeneration Expression 17. In-Situ Hybridization 18. ChIP-seq for ADAMTS1 & 6 --- Section Divider --- Summary 19. Key Findings Summary --- Section Divider --- Biotech Experience 20. GAIA Biotechnologies 21. EFA Technologies 22. Red C Biotech --- Section Divider --- Bollyky Lab Work 23. Phage Biology & Gene Delivery 24. Preliminary Results 25. Thank You / Questions Enhance slide clarity by: - Converting dense paragraphs to structured bullet points - Adding bold takeaway statements to data-heavy slides - Improving hierarchy and spacing - Ensuring visual consistency throughout Make the presentation conference-ready and visually striking.
This presentation details the ADAMTS gene family in the cnidarian model Nematostella vectensis, covering its identification, phylogenetics, domain architecture, expression during development and regeneration, key findings on basal origins, and the现er
February 19, 202621 slides
Slide 2 of 21
Slide 2 - Themes of Presentation
- Nematostella as a model organism
- Characterization of Nv-ADAMTS genes
- Phylogenetics + domain architecture
- Development & regeneration expression
- Focus on Nv-ADAMTS1, 6, 14, 19
Slide 3 of 21
Slide 3 - Nematostella vectensis: A Basal Metazoan Model
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Nematostella vectensis: A Basal Metazoan Model
A cnidarian model organism for studying evolution, regeneration, and development
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Slide 4 of 21
Slide 4 - Life Cycle & Morphology
- Sessile polyp form with radial symmetry
- Mouth surrounded by tentacles bearing cnidocytes for prey capture
- Habitat: shallow brackish lagoons, column buried in mud
- Cnidaria phylum: basal metazoans, sister group to Bilateria
- Genome sequenced; key lab model for genetics & evolution
Slide 5 of 21
Slide 5 - Whole-Body Regeneration & Experimental Tools
- Remarkable regeneration: reforms from small body fragments
- Asexual reproduction via fission
- Pluripotent cells enable regeneration
- Lab tools: CRISPR genome editing, RNA-seq, in-situ hybridization, ChIP-seq
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Slide 6 of 21
Slide 6 - ADAMTS Proteases Background
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ADAMTS Proteases Background
Understanding modular structure, clades, and roles in cnidarians
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Slide 7 of 21
Slide 7 - ADAMTS Modular Structure
- Multi-domain secreted metalloproteases
- Key domains: Pro (activation), Metalloprotease (catalytic), Disintegrin (adhesion)
- Thrombospondin repeats (TS1-5): substrate binding
- C-terminal ancillary domains: specificity & localization
- Takeaway: Highly conserved modular architecture across metazoans
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Slide 8 of 21
Slide 8 - ADAMTS Clades & Vertebrate Functions
- ~19 clades in vertebrates
- Diverse functions: ECM remodeling, angiogenesis, blood coagulation
- Aggrecanases (ADAMTS4/5): cartilage degradation
- Cnidarian ADAMTS: Basal origins unclear
- Open questions: Roles in regeneration? Conservation in non-bilaterians?
Slide 9 of 21
Slide 9 - Nv-ADAMTS Family Analysis
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Nv-ADAMTS Family Analysis
Identifying and characterizing homologues in N. vectensis
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Slide 10 of 21
Slide 10 - Identifying Nv-ADAMTS Homologues & Motif Conservation
- Homology search: Reciprocal BLAST, HMM profiles vs. human ADAMTS
- ~22 Nv-ADAMTS genes identified
- Core domains conserved: Metalloprotease, Disintegrin, TS1
- Ancillary domains variable
- Takeaway: Ancient family predating bilaterians
Slide 11 of 21
Slide 11 - Phylogenetic Pipeline
| Step | Description |
|---|---|
| 1. Homologue collection | BLAST/HMMER across metazoans |
| 2. Multiple sequence alignment | MAFFT or MUSCLE |
| 3. Model selection | ProtTest / IQ-TREE |
| 4. Tree inference | RAxML / IQ-TREE maximum likelihood |
| 5. Clade annotation | Reference vertebrate clades |
Slide 12 of 21
Slide 12 - Shifting ADAMTS Clade Origins
- Nv-ADAMTS reveal basal origins for key clades
- Shifts in clade assignments vs. vertebrate-only trees
- Ancestral duplications predate cnidarian-bilaterian split
- Key finding: Cnidarian roots reshape ADAMTS evolution
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Slide 13 of 21
Slide 13 - Expression Analysis
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Expression Analysis
Developmental, spatial, and regeneration expression of Nv-ADAMTS genes
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Slide 14 of 21
Slide 14 - Spatiotemporal Expression Experiments
- qPCR / RNA-seq across dev stages & regeneration timecourse
- Development: Maternal to planula, polyp stages
- Regeneration: Upregulation peaks at 24-48h post-amputation
- Dynamic expression suggests roles in tissue remodeling
- Focus genes: Nv-ADAMTS1, 6, 14, 19
Slide 15 of 21
Slide 15 - Spatial Expression Patterns
- Enriched in specific tissues: e.g., Nv-ADAMTS1 in mesenteries
- Regeneration-specific domains upregulated
- Takeaway: Tissue-specific roles in homeostasis & repair
Slide 16 of 21
Slide 16 - In-Situ Hybridization & ChIP-seq
- ISH: Localized expression in endoderm/mesoglea
- ChIP-seq: Regulatory elements bound by key TFs
- Validates RNA-seq; reveals cis-regulatory logic
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Slide 17 of 21
Slide 17 - Key Findings & Summary
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Key Findings Summary
ADAMTS evolution and function in a basal metazoan
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Slide 18 of 21
Slide 18 - Key Findings
- Nv-ADAMTS family ancient, predates bilaterians
- Shifts in phylogenetic clade assignments
- Tissue-specific expression in development & regeneration
- Upregulation during regeneration suggests ECM remodeling roles
- Nv-ADAMTS1/6: Prime candidates for functional studies
Slide 19 of 21
Slide 19 - Biotech Experience
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Biotech Experience
Industry background in biotechnology startups
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Slide 20 of 21
Slide 20 - Previous Roles
🧬 GAIA Biotechnologies Synthetic biology & gene editing platforms
🔬 EFA Technologies Enzyme engineering for therapeutics
💉 Red C Biotech Cell therapy & regenerative medicine
Slide 21 of 21
Slide 21 - Bollyky Lab Work & Thank You
Phage Biology & Gene Delivery Preliminary Results Promising
Thank You! Questions?
Joining Bollyky Lab to explore phage-based delivery for ADAMTS modulation
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